ACHE ELISA Kit (Human) (OKCD07400)

Catalog No: OKCD07400

Size:96 Wells

ACHE ELISA Kit (Human) (OKCD07400) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ACHE ELISA Kit (Human) (OKCD07400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACHE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACHE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACHE captured in the well.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level ACHE. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level ACHE using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • ACHE Microplate: 96 Wells (12 x 8 Well strips)
  • ACHE Lyophilized Standard: 2
  • 100X ACHE HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 1.21ng/mL

Target Info

• Gene Symbol: ACHE
• Gene Full Name: acetylcholinesterase (Cartwright blood group)
• Alias Symbols: YT, ACEE, ARACHE, N-ACHE
• NCBI Gene Id: 43
• Protein Name: Acetylcholinesterase
• Description of Target: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood gro
• Uniprot ID: P22303
• Protein Accession #: NP_000656.1
• Nucleotide Accession #: NM_000665.4