ACHE ELISA Kit (Human) (OKCD07400)

Catalog No: OKCD07400

Size:96WELLS

ACHE ELISA Kit (Human) (OKCD07400) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetylcholinesterase (ACHE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetylcholinesterase (ACHE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetylcholinesterase (ACHE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Acetylcholinesterase (ACHE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetylcholinesterase (ACHE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetylcholinesterase (ACHE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ACHE Microplate: 96 Wells (12 x 8 Well strips)
  • ACHE Lyophilized Standard: 2
  • 100X ACHE HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 1.21ng/mL
• Description:
  View product citations for ACHE ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: ACHE
• Gene Full Name: acetylcholinesterase (Cartwright blood group)
• Alias Symbols: ACEE;acetylcholinesterase;acetylcholinesterase (Yt blood group);apoptosis-related acetylcholinesterase;ARACHE;N-ACHE;YT;Yt blood group.
• NCBI Gene Id: 43
• Protein Name: Acetylcholinesterase
• Description of Target: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
• Uniprot ID: P22303
• Protein Accession #: NP_000656.1
• Nucleotide Accession #: NM_000665.4