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Catalog No: OKEH02568
Size:96T
Price: $600.00
SKU
OKEH02568
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02568
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Acetylcholine ELISA Kit (OKEH02568) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Acetylcholine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Acetylcholine and incubated. The Acetylcholine found in the sample or standards competes with the biotinylated Acetylcholine for limited binding sites on the immobilized anti-Acetylcholine antibody. Excess unbound biotinylated Acetylcholine and sample or standard Acetylcholine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Acetylcholine captured in the well and inversely proportional to the amount of Acetylcholine which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Acetylcholine Microplate96 Wells (12 x 8 Well strips)
Acetylcholine Lyophilized Standard2 x 5,000 pg
Sample Diluent1 x 20 mL
100X Acetylcholine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: ACH
Target Name: Acetylcholine
::Pubchem: 187
::Chemical formula: C7H16NO2+
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity24 pg/mL
SpecificityNatural and recombinant General Acetylcholine
Publications

Effects of Topical Gabapentin on Ocular Pain and Tear Secretion. Front Pharmacol. 12, 671238 (2021). 34163358

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsACH, Acetylcholine, ACH
Protein NameAcetylcholine
Description of TargetAcetylcholine has functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS) as a neuromodulator. Its receptors have very high binding constants. In the peripheral nervous system, acetylcholine activates muscles, and is a major neurotransmitter in the autonomic nervous system. In the central nervous system, acetylcholine and the associated neurons form a neurotransmitter system, the cholinergic system, which tends to cause anti-excitatory actions.
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