Acetylcholine ELISA Kit (OKEH02568)

Catalog No: OKEH02568

Size:96 Wells

Acetylcholine ELISA Kit (OKEH02568) standard curve.

Product Info

• Predicted Species Reactivity: All
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Acetylcholine ELISA Kit (OKEH02568) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Acetylcholine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Acetylcholine and incubated. The Acetylcholine found in the sample or standards competes with the biotinylated Acetylcholine for limited binding sites on the immobilized anti-Acetylcholine antibody. Excess unbound biotinylated Acetylcholine and sample or standard Acetylcholine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Acetylcholine captured in the well and inversely proportional to the amount of Acetylcholine which was contained in the sample or standard.
• ELISA Kit Range: 78 - 5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 92%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.4% (n = 20)
  Mean Inter-assay CV%: < 8.1% (n = 20)
• ELISA Kit Component:
  • Anti-Acetylcholine Microplate: 96 Wells (12 x 8 Well strips)
  • Acetylcholine Lyophilized Standard: 2 x 5,000 pg
  • Sample Diluent: 1 x 20 mL
  • 100X Acetylcholine-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: ACH
  Target Name: Acetylcholine
  Pubchem: 187
  Chemical formula: C7H16NO2+
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 24 pg/mL
• Specificity: Natural and recombinant General Acetylcholine
• Publications:

  Effects of Topical Gabapentin on Ocular Pain and Tear Secretion. Front Pharmacol. 12, 671238 (2021). 34163358

• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: ACH, Acetylcholine, ACH
• Protein Name: Acetylcholine
• Description of Target: Acetylcholine has functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS) as a neuromodulator. Its receptors have very high binding constants. In the peripheral nervous system, acetylcholine activates muscles, and is a major neurotransmitter in the autonomic nervous system. In the central nervous system, acetylcholine and the associated neurons form a neurotransmitter system, the cholinergic system, which tends to cause anti-excitatory actions.