Catalog No: OPCA335985
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OPCA335985
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ACE2 Recombinant Protein (Human) (OPCA335985)

Datasheets/ManualsPrintable datasheet for OPCA335985
Product Info
Predicted Species ReactivityHuman
Product FormatLyophilized powder
Additional InformationBiological Activity:
1. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2ug/well (2ug/ml, 100ul/well) of SARS-CoV-2 S1 protein , add 100ul/well (0.7629-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml.
2. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5ug/well (5ug/ml, 100ul/well) of SARS-CoV-2 S1-RBD protein , add 100ul/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml.
3. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5ug/well (5ug/ml, 100ul/well) of SARS-CoV-2 S1-RBD protein , add 100ul/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml.
4. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2ug/well (2ug/ml, 100ul/well) of ACE2 protein, add 100ul/well (0.00001164153-200000ng/ml, gradient dilution, 4x to 18x) of SARS-CoV-2 S1-RBD protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml.
5. The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations (400, 200, 100, 50, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM.
6. The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations (100, 50, 25, 12.5, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.
::Biological Activity: 1. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2ug/well (2ug/ml, 100ul/well) of SARS-CoV-2 S1 protein , add 100ul/well (0.7629-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml. 2. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5ug/well (5ug/ml, 100ul/well) of SARS-CoV-2 S1-RBD protein , add 100ul/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml. 3. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5ug/well (5ug/ml, 100ul/well) of SARS-CoV-2 S1-RBD protein , add 100ul/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml. 4. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2ug/well (2ug/ml, 100ul/well) of ACE2 protein, add 100ul/well (0.00001164153-200000ng/ml, gradient dilution, 4x to 18x) of SARS-CoV-2 S1-RBD protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml. 5. The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations (400, 200, 100, 50, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM. 6. The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations (100, 50, 25, 12.5, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
PurificationAffinity purified using IMAC
PurityGreater than 85% as determined by SDS-PAGE.
Biological Activity1. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2ug/well (2ug/ml, 100ul/well) of SARS-CoV-2 S1 protein , add 100ul/well (0.7629-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml. 2. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5ug/well (5ug/ml, 100ul/well) of SARS-CoV-2 S1-RBD protein , add 100ul/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml. 3. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5ug/well (5ug/ml, 100ul/well) of SARS-CoV-2 S1-RBD protein , add 100ul/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml. 4. Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2ug/well (2ug/ml, 100ul/well) of ACE2 protein, add 100ul/well (0.00001164153-200000ng/ml, gradient dilution, 4x to 18x) of SARS-CoV-2 S1-RBD protein and 100ul/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific . Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml. 5. The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations (400, 200, 100, 50, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM. 6. The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein . ACE2 protein was injected at 5 concentrations (100, 50, 25, 12.5, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM.
Protein SequenceQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS
Storage BufferLyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
SourceMammalian cell
Protein Range18-740 aa
TagC-terminal hFc-tagged
Gene SymbolACE2
Gene Full Nameangiotensin I converting enzyme 2
Protein NameAngiotensin-converting enzyme 2
Description of TargetAngiotensin-converting enzyme 2 (ACE2), also known as ACAH, is an essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system, which is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. ACE2 is a suppressor of renin-angiotensin system (RAS). ACE2 converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, a vasodilator. Besides the role in the cardiovascular system, ACE2 also plays a role in lung diseases as a receptor for SARS-CoV-2. Recently, with the COVID19 caused by SARS-CoV-2 spreads around the world, ACE2 functions as a receptor on the cell surface to bind with spike protein of SARS-CoV-2 in SARS-CoV-2 infection. And the mechanism of SARS-CoV-2 invasion via ACE2 are gradually being revealed. Based on these studies, there are more coronavirus-host interactome targets which have been found, such as TMPRSS2 and CD147. All of these works are critical to SARS-CoV-2 treatment, and provide potential targets of drug development for SARS-CoV-2 therapy.
Swissprot IdQ9BYF1
Protein Size (# AA)18-740 aa
Molecular Weight112.5 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express ACE2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express ACE2.
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