Catalog No: OKCD07769
Size:96 Wells
Price: $825.00
SKU
OKCD07769
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACE2 ELISA Kit (Rat) (OKCD07769) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACE2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACE2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACE2 captured in the well.
ELISA Kit Range0.781-50ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level ACE2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level ACE2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
ACE2 Microplate96 Wells (12 x 8 Well strips)
ACE2 Lyophilized Standard2
100X ACE2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.31ng/mL
Gene SymbolACE2
Gene Full Nameangiotensin I converting enzyme 2
Alias SymbolsACEH; ACEII; ACE-II; Peptidyl-Dipeptidase A; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15
NCBI Gene Id302668
Protein Nameangiotensin-converting enzyme 2
Description of Targetenzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels.
Swissprot IdQ5EGZ1
Protein Accession #NP_001012006.1
Nucleotide Accession #NM_001012006.1
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express ACE2.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express ACE2.
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