ACE2 ELISA kit (Rat) (OKCD07769)

Catalog No: OKCD07769

Size:96WELLS

ACE2 ELISA Kit (Rat) (OKCD07769) Standard Curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme 2 (ACE2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiotensin I Converting Enzyme 2 (ACE2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ACE2 Microplate: 96 Wells (12 x 8 Well strips)
  • ACE2 Lyophilized Standard: 2
  • 100X ACE2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.31ng/mL
• Description:
  View product citations for ACE2 ELISA kit (Rat) on CiteAb

Target Info

• Gene Symbol: Ace2
• Gene Full Name: angiotensin I converting enzyme 2
• Alias Symbols: ACE-related carboxypeptidase;angiotensin I converting enzyme (peptidyl-dipeptidase A) 2;angiotensin-converting enzyme 2;anigotensin-converting enzyme-related carboxypeptidase;renal angiotensin-converting enzyme 2.
• NCBI Gene Id: 302668
• Protein Name: Q5EGZ1
• Description of Target: Angiotensin-converting enzyme 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q5EGZ1
• Protein Accession #: NP_001012006.1
• Nucleotide Accession #: NM_001012006.1