Catalog No: OKEH01348
Size:96 Wells
Price: $525.00
SKU
OKEH01348
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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ACE2 ELISA Kit (Rat) : 96 Wells (OKEH01348)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01348
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACE2 ELISA Kit (Rat) (OKEH01348) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACE2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACE2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACE2 captured in well.
ELISA Kit Range0.312 - 20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
ACE2 Microplate96 Wells (12 x 8 Well strips)
ACE2 Lyophilized Standard2
100X Biotinylated ACE2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.091 ng/mL
SpecificityNatural and recombinant Rat Angiotensin-converting enzyme 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolACE2
Gene Full Nameangiotensin I converting enzyme 2
Alias SymbolsACE-related carboxypeptidase, Angiotensin-converting enzyme 2
NCBI Gene Id302668
Protein NameAngiotensin-converting enzyme 2
Description of Targetenzyme peptidyl-dipeptidase; mouse homolog has a role in regulation of cardiac contractility and regulating Angiotensin 1 and 2 levels 
Swissprot IdQ5EGZ1
Protein Accession #NP_001012006.1
Nucleotide Accession #NM_001012006.1
Protein Size (# AA)805
Molecular Weight92 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express ACE2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express ACE2.
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