Catalog No: OKEH06836
Size:96 Wells
Price: $525.00
SKU
OKEH06836
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH06836
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ace2 ELISA Kit (Mouse) (OKEH06836) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ace2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ace2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ace2 captured in the well.
ELISA Kit Range0.312 - 20ng/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 86%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <7.9%
Inter-Assay CV <10.3%
ELISA Kit Component
ComponentAmount
Ace2 Microplate96 Wells (12 x 8 Well strips)
Ace2 Lyophilized Standard2 x 20 ng
100X Ace2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.174 ng/mL
SpecificityNatural and recombinant Mouse Angiotensin-converting enzyme 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolACE2
Alias Symbols2010305L05Rik
NCBI Gene Id70008
Protein NameAngiotensin-converting enzyme 2
Description of TargetCarboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. May have a protective role in acute lung injury.
Swissprot IdQ8R0I0
Protein Accession #NP_001123985
Nucleotide Accession #NM_001130513
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express ACE2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express ACE2.
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