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Catalog No: OKCD07768
Size:96WELLS
Price: $650.00
SKU
OKCD07768
Availability: Domestic: within 2-3 weeks delivery | International: within 2-3 weeks delivery
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Datasheets/ManualsPrintable datasheet for ACE2 ELISA Kit (Mouse) (OKCD07768)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme 2 (ACE2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme 2 (ACE2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiotensin I Converting Enzyme 2 (ACE2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range62.5-4,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme 2 (ACE2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ACE2 Microplate96 Wells (12 x 8 Well strips)
ACE2 Lyophilized Standard2
100X ACE2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 5.7pg/mL
Gene SymbolAce2
Gene Full Nameangiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Alias Symbols2010305L05Rik;ACE-related carboxypeptidase;angiotensin I converting enzyme 2;angiotensin-converting enzyme 2.
NCBI Gene Id70008
Protein NameAngiotensin-converting enzyme 2
Description of TargetCarboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. May have a protective role in acute lung injury.
Uniprot IDQ8R0I0
Protein Accession #NP_001123985.1
Nucleotide Accession #NM_001130513.1
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