Catalog No: OKBB00649
Size:96 Wells
Price: $425.00
SKU
OKBB00649
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human ACE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ACE2 has been precoated onto 96-well plates. Standards(NS0, Q18-S740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ACE2 amount of sample captured in plate.
ELISA Kit Range62.5 pg/ml - 4,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)4381175238650812932417
SD19.7156.4124.0734.5498.27188.53
CV%4.54.85.26.87.67.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human ACE2 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human ACE2 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human ACE2 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human ACE2
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: Q18-S740
DescriptionFor quantitative detection of human ACE2 in cell culture supernatants, serum and plasma(heparin).
Gene SymbolACE2
Gene Full Nameangiotensin I converting enzyme 2
Alias SymbolsACE 2, ACE related carboxypeptidase, ACE-related carboxypeptidase, ACE2, ACE2_HUMAN, ACEH, Angiotensin converting enzyme 2, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZP434A014, EC 3.4.17, metalloprotease MPROT 15, Metalloprotease MPROT15, OTTHUMP00000022963, Processed angiotensin-converting enzyme 2
NCBI Gene Id59272
Protein NameAngiotensin-converting enzyme 2
Description of TargetAngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Swissprot IdQ9BYF1
Protein Accession #NP_068576.1
Nucleotide Accession #NM_021804.2
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express ACE2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express ACE2.
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