ACE2 ELISA Kit (Human) (OKBB00649)

Catalog No: OKBB00649

Size:96 Wells

Human ACE2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human ACE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ACE2 has been precoated onto 96-well plates. Standards(NS0, Q18-S740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ACE2 amount of sample captured in plate.
• ELISA Kit Range: 62.5 pg/ml - 4,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	438	1175	2386	508	1293	2417
  SD	19.71	56.4	124.07	34.54	98.27	188.53
  CV%	4.5	4.8	5.2	6.8	7.6	7.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human ACE2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human ACE2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human ACE2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human ACE2
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q18-S740
• Description: For quantitative detection of human ACE2 in cell culture supernatants, serum and plasma(heparin).

Target Info

• Gene Symbol: ACE2
• Gene Full Name: angiotensin I converting enzyme 2
• Alias Symbols: ACE 2, ACE related carboxypeptidase, ACE-related carboxypeptidase, ACE2, ACE2_HUMAN, ACEH, Angiotensin converting enzyme 2, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZP434A014, EC 3.4.17, metalloprotease MPROT 15, Metalloprotease MPROT15, OTTHUMP00000022963, Processed angiotensin-converting enzyme 2
• NCBI Gene Id: 59272
• Protein Name: Angiotensin-converting enzyme 2
• Description of Target: AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
• Uniprot ID: Q9BYF1
• Protein Accession #: NP_068576.1
• Nucleotide Accession #: NM_021804.2