ACE2 Antibody (OAPB00219)

Catalog No: OAPB00219

Size:0.1 mg

Figure 2 Western Blot Validation in Human Tissues
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Western Blot Validation in Mouse Tissues
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 4 Western Blot Validation in Rat Thymus Tissue
Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues (kidney, spleen testis, breast, intestine)
Loading: 15 ug of lysates per lane. Antibodies: OAPB00213 (2 ug/mL), OAPB00214 (2 ug/mL), OAPB00219 (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 5 Immunohistochemistry Validation of ACE2 in Human Kidney Tissue
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Figure 6 Immunofluorescence Validation of ACE2 in Human Kidney Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney cells labeling ACE2 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS with 0.02% sodium azide
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: ELISA, WB, IHC-P, IF
• Additional Information: Positive Control: Human Kidney, Human Spleen
• Reconstitution and Storage: Stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-ACE2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human ACE2.
  The immunogen is located within the first 50 amino acids of ACE2.
• Purification: ACE2 Antibody is affinity chromatography purified via peptide column.
• Predicted Homology Based on Immunogen Sequence: Predicted species reactivity based on immunogen sequence: Bovine: (86%)
• Concentration: 1 mg/mL
• Blocking Peptide: For anti-ACE2 antibody is Catalog # OEPB02313
• Specificity: Anti-ACE2 has no cross response to ACE1.
• Application Info: WB: 1-2 ug/mL
  IHC: 2 ug/mL
  IF: 10 ug/mL. Antibody validated: WB in human, mouse and rat samples; IHC in human samples; IF in human, mouse and rat samples.
• Reference: Donoghue et al. Circ. Res. 2000;87:1-9.
  Tipnis et al. J Biol. Chem. 2000;275:33238-43.
  Li et al. Nature 2003;426:450-4.
  Lu et al. The Lancet 2020 (published online).
  Crackower et al. Nature 2002;417:822-8.

Target Info

• Gene Symbol: ACE2
• Gene Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Alias Symbols: ACEH
• NCBI Gene Id: 59272
• Protein Name: Angiotensin-converting enzyme 2
• Description of Target: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
• Uniprot ID: Q9BYF1
• Protein Accession #: NP_068576
• Molecular Weight: Predicted: 93kDa
  Observed: 130 kDa (7 N-linked glycosylation)