ACE2 Antibody - middle region (OASG00144)

Catalog No: OASG00144

Size:100 uL

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: WB, ELISA
• Additional Information: Cellular Localization: Processed angiotensin-converting enzyme 2: Secreted.; Cell membrane; Single-pass type I membrane protein. Cytoplasm. Detected in both cell membrane and cytoplasm in neurons.
  Tissue Specificity: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from the Internal region of human ACE2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1.
• Specificity: ACE2 Polyclonal Antibody detects endogenous levels of ACE2 protein.
• Application Info: WB: 1:500-1:2000
  ELISA: 1:40,000
  Optimal dilutions should be determined by the end user.
• Publications:

  Activation of Kinin B1R Upregulates ADAM17 and Results in ACE2 Shedding in Neurons. Int J Mol Sci. 22, (2020). 33375653

  Expression of ACE2 in Human Neurons Supports the Neuro-Invasive Potential of COVID-19 Virus. Cell Mol Neurobiol. NULL, NULL (2020). 32623546

• Description:
  View product citations for ACE2 Antibody - middle region (OASG00144) on CiteAb

Target Info

• Gene Symbol: ACE2
• Gene Full Name: angiotensin I converting enzyme 2
• Alias Symbols: ACEH
• NCBI Gene Id: 59272; 70008;
• Description of Target: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. (Microbial infection) Acts as a receptor for SARS coronavirus/SARS-CoV and human coronavirus NL63/HCoV-NL63.
• Uniprot ID: Q9BYF1, Q8R0I0
• Molecular Weight: 90 kDa