ACE ELISA Kit (Pig) (OKCD00735)

Catalog No: OKCD00735 (Formerly GWB-KBBER6)

Size:96WELLS

Pig ACE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-103%	78-102%	85-103%	82-93%
  EDTA plasma(n=5)	96-105%	82-101%	95-104%	78-92%
  heparin plasma(n=5)	78-89%	97-104%	93-102%	99-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiotensin I Converting Enzyme (ACE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Angiotensin I Converting Enzyme (ACE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	96-103	101
  heparin plasma(n=5)	78-105	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ACE Microplate: 96 Wells (12 x 8 Well Strips)
  • ACE Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated ACE Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 11.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE). No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ACE
• Alias Symbols: CD143, ACE1, DCP1, ACEI, ACE-I, Kininase II, Angiotensin-Converting Enzyme, Peptidyl-Dipeptidase A, Dipeptidyl Carboxypeptidase 1, Angiotensin-converting enzyme, soluble form
• NCBI Gene Id: 613133
• Protein Name: Angiotensin-converting enzyme
• Description of Target: Angiotensin-converting enzyme
• Uniprot ID: F1RRW5
• Protein Accession #: NP_001028187
• Nucleotide Accession #: NM_001033015.2
• Protein Size (# AA): 745