ACE ELISA Kit (Mouse) (OKBB00364)

Catalog No: OKBB00364

Size:96 Wells

Mouse ACE ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ACE has been precoated onto 96-well plates. Standards(NS0, L35-Q1264) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse ACE amount of sample captured in plate.
• ELISA Kit Range: 93.8 pg/ml - 6,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	0.64	1.98	4.25	0.85	2.02	5.32
  SD	0.029	0.085	0.179	0.062	0.141	0.367
  CV%	4.6	4.3	4.2	7.3	7	6.9

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse ACE Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse ACE Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse ACE Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse ACE
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L35-Q1264
• Description: For quantitative detection of mouse ACE in cell culture supernatants, serum and plasma(heparin).

Target Info

• Gene Symbol: ACE
• Gene Full Name: angiotensin I converting enzyme
• Alias Symbols: ACE 1, ACE T, Angiotensin converting enzyme, Angiotensin Converting Enzyme 1, Angiotensinconverting enzyme, Angiotensin-converting enzyme, CD143 antigen, DCP 1, DCP, DCP1, Dipeptidyl carboxypeptidase 1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3, P12821, Peptidyl dipeptidase A
• NCBI Gene Id: 11421
• Protein Name: Angiotensin-converting enzyme
• Description of Target: Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II. Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic' and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.
• Uniprot ID: P09470
• Protein Accession #: NP_033728.1
• Nucleotide Accession #: NM_009598.2