Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD03277
Size:96WELLS
Price: $850.00
SKU
OKCD03277
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

ACE Chemi-Luminescent ELISA Kit (Mouse) (OKCD03277)

Datasheets/ManualsPrintable datasheet for ACE Chemi-Luminescent ELISA Kit (Mouse) (OKCD03277)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)78-95%79-96%86-101%94-101%
EDTA Plasma (n=5)95-103%95-102%94-104%78-97%
Heparin Plasma (n=5)96-104%80-102%96-104%82-91%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensin I Converting Enzyme (ACE) level in the sample or standard.
ELISA Kit Range54.9-40,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)98-105101
EDTA Plasma (n=5)95-105102
Heparin Plasma (n=5)98-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ace Microplate96 Wells (12 x 8 Well strips)
Ace Lyophilized Standard2
100X Ace Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cerebrospinal fluid, cell lysates, cell culture supernates and other biological fluids
Sensitivity54.9 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAce
Gene Full Nameangiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Alias Symbolsangiotensin-converting enzyme;AW208573;CD143;dipeptidyl carboxypeptidase I;dipeptidyl peptidase;kininase II.
NCBI Gene Id11421
Protein NameAngiotensin-converting enzyme
Description of TargetConverts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.
Uniprot IDP09470
Protein Accession #NP_033728
Nucleotide Accession #NM_009598
Write Your Own Review
You're reviewing:ACE Chemi-Luminescent ELISA Kit (Mouse) (OKCD03277)
Your Rating
We found other products you might like!