ACE Chemi-Luminescent ELISA Kit (Human) (OKCD03276)

Catalog No: OKCD03276

Size:96WELLS

Human ACE ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-98%	81-97%	89-103%	89-103%
  EDTA Plasma (n=5)	95-105%	94-102%	78-99%	80-88%
  Heparin Plasma (n=5)	80-105%	85-99%	89-98%	89-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Angiotensin I Converting Enzyme (ACE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensin I Converting Enzyme (ACE) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-96	92
  EDTA Plasma (n=5)	83-101	92
  Heparin Plasma (n=5)	82-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ACE Microplate: 96 Wells (12 x 8 Well strips)
  • ACE Lyophilized Standard: 2
  • 100X ACE Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 68.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACE
• Gene Full Name: angiotensin I converting enzyme
• Alias Symbols: ACE1;angiotensin I converting enzyme (peptidyl-dipeptidase A) 1;angiotensin-converting enzyme;carboxycathepsin;CD143;CD143 antigen;DCP;DCP1;dipeptidyl carboxypeptidase 1;dipeptidyl carboxypeptidase I;kininase II;peptidase P.
• NCBI Gene Id: 1636
• Protein Name: Angiotensin-converting enzyme
• Description of Target: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
• Uniprot ID: P12821
• Protein Accession #: NP_000780
• Nucleotide Accession #: NM_000789