ACD ELISA Kit (Human) (OKEI00126)

Catalog No: OKEI00126

Size:96 Wells

Human ACD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ACD ELISA Kit (Human) (OKEI00126) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACD has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample ACD
• ELISA Kit Range: 0.031 - 2 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level ACD. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level ACD using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • ACD Microplate: 96 Wells (12 x 8 Well strips)
  • ACD Lyophilized Standard: 2
  • 100X ACD HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 30X Wash Buffer: 1 x 30 mL
  • 100X Luminol Substrate: 1 x 10 mL
  • Substrate Diluent: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 18.75 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACD
• Alias Symbols: PIP1, PTOP, TPP1, TINT1
• NCBI Gene Id: 65057
• Protein Name: Adrenocortical dysplasia protein homolog
• Description of Target: Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends. Without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomere elongation by recruiting telomerase to telomeres and increasing its processivity. May play a role in organogenesis.
• Uniprot ID: Q96AP0
• Protein Accession #: NP_001075955
• Nucleotide Accession #: NM_001082486