ACCN2 Antibody - C-terminal region (OAAB05862)

Catalog No: OAAB05862

Size:400ul

Western blot analysis of ACCN2 Antibody (C-term) in CEM cell lysates (35ug/lane). ACCN2 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ACCN2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

ACCN2 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC, IHC-P
• Additional Information: Function: Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
  Cellular Location Cell membrane; Multi- pass membrane protein Note=Localizes in synaptosomes at dendritic synapses of neurons Colocalizes with DLG4 (By similarity).
  Tissue Location Expressed in most or all neurons.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ACCN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-526 amino acids from the C-terminal region of human ACCN2.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Chicken, Mouse, Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: ACCN2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
• Other Applications Image 1 Data: ACCN2 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: ACCN2
• Alias Symbols: ASIC, ACCN2, BNaC2
• NCBI Gene Id: 41
• Protein Name: Acid-sensing ion channel 1
• Description of Target: ACCN2 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member is expressed in most if not all brain neurons, and it may be an ion channel subunit; however, its function as an ion channel remains unknown.
• Uniprot ID: P78348
• Protein Accession #: NP_001086.2
• Molecular Weight: 60 kDa