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Catalog No: OKCD02302
Size:96WELLS
Price: $700.00
SKU
OKCD02302
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Datasheets/ManualsPrintable datasheet for ACAN ELISA Kit (Rat) (OKCD02302)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)84-101%79-89%87-101%97-105%
EDTA Plasma (n=5)92-101%80-96%83-101%78-90%
Heparin Plasma (n=5)84-103%83-101%81-98%96-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aggrecan (AGC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aggrecan (AGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aggrecan (AGC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aggrecan (AGC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)92-10196
EDTA Plasma (n=5)80-9790
Heparin Plasma(n=5)84-9587
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aggrecan (AGC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aggrecan (AGC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ACAN Microplate96 Wells (12 x 8 Well strips)
ACAN Lyophilized Standard2
100X Biotinylated ACAN Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.137 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aggrecan (AGC). No significant cross-reactivity or interference between Aggrecan (AGC) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAcan
Gene Full Nameaggrecan
Alias SymbolsAgc;Agc1;aggrecan 1;aggrecan core protein;aggrecan, structural proteoglycan of cartilage;cartilage-specific proteoglycan core protein;CSPCP.
NCBI Gene Id58968
Protein NameAggrecan core protein
Description of TargetThis proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
Uniprot IDP07897
Protein Accession #NP_071526.1
Nucleotide Accession #NM_022190.1
Protein Size (# AA)2124
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