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Catalog No: OKEH00570 (Formerly GWB-QB5FEH)
Size:96T
Price: $525.00
SKU
OKEH00570
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ACAN ELISA Kit (Human) (OKEH00570) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human ACAN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human ACAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human ACAN captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.8% (n = 20)
Mean Inter-assay CV%: < 11.6% (n = 20)
ELISA Kit Component
ComponentAmount
ACAN Microplate96 Wells (12 x 8 Well strips)
ACAN Lyophilized Standard2
100X Biotinylated ACAN Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.23 ng/mL
SpecificityNatural and recombinant Human Aggrecan core protein
Publications

A comparative proteomic study of plasma in Colombian childhood acute lymphoblastic leukemia. PLoS One. 14, e0221509 (2019). 31437251

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolACAN
Gene Full Nameaggrecan
Alias SymbolsAGC1;AGCAN;aggrecan core protein;cartilage-specific proteoglycan core protein;chondroitin sulfate proteoglycan core protein 1;CSPG1;CSPGCP;large aggregating proteoglycan;MSK16;SEDK;SSOAOD.
NCBI Gene Id176
Protein NameP16112
Description of TargetAggrecan core protein
Uniprot IDhttps://www.uniprot.org/uniprot/P16112
Protein Accession #NP_001126.3
Nucleotide Accession #NM_001135.3
Protein Size (# AA)2415
Molecular Weight250 kDa
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