ACAN ELISA Kit (Human) : 96 Wells (OKEH00570)

Catalog No: OKEH00570 (Formerly GWB-QB5FEH)

Size:96 Wells

Human ACAN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ACAN ELISA Kit (Human) (OKEH00570) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human ACAN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human ACAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human ACAN captured in well.
• ELISA Kit Range: 0.78 - 50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.8% (n = 20)
  Mean Inter-assay CV%: < 11.6% (n = 20)
• ELISA Kit Component:
  • ACAN Microplate: 96 Wells (12 x 8 Well strips)
  • ACAN Lyophilized Standard: 2
  • 100X Biotinylated ACAN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Human Aggrecan core protein
• Publications:

  A comparative proteomic study of plasma in Colombian childhood acute lymphoblastic leukemia. PLoS One. 14, e0221509 (2019). 31437251

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACAN
• Gene Full Name: aggrecan
• Alias Symbols: AGC1, SEDK, AGCAN, CSPG1, MSK16, CSPGCP, SSOAOD
• NCBI Gene Id: 176
• Protein Name: Aggrecan core protein
• Description of Target: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
• Uniprot ID: P16112
• Protein Accession #: NP_001126.3
• Nucleotide Accession #: NM_001135.3
• Protein Size (# AA): 2415
• Molecular Weight: 250 kDa