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Catalog No: OKCD03284
Size:96WELLS
Price: $800.00
SKU
OKCD03284
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ACAN Chemi-Luminescent ELISA Kit (Human) (OKCD03284)

Datasheets/ManualsPrintable datasheet for ACAN Chemi-Luminescent ELISA Kit (Human) (OKCD03284)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-99%98-105%93-101%87-95%
EDTA Plasma (n=5)80-104%79-102%94-101%92-105%
Heparin Plasma (n=5)91-102%81-101%79-89%78-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Aggrecan (AGC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Aggrecan (AGC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Aggrecan (AGC) level in the sample or standard.
ELISA Kit Range41.2-30,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)91-9996
EDTA Plasma (n=5)82-8986
Heparin Plasma (n=5)92-10195
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aggrecan (AGC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aggrecan (AGC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ACAN Microplate96 Wells (12 x 8 Well strips)
ACAN Lyophilized Standard2
100X ACAN Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.47 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolACAN
Gene Full Nameaggrecan
Alias SymbolsAGC1;AGCAN;aggrecan core protein;cartilage-specific proteoglycan core protein;chondroitin sulfate proteoglycan core protein 1;CSPG1;CSPGCP;large aggregating proteoglycan;MSK16;SEDK;SSOAOD.
NCBI Gene Id176
Protein NameAggrecan core protein
Description of TargetThis proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
Uniprot IDP16112
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