ACACA ELISA Kit (Human) (OKCD02337)

Catalog No: OKCD02337

Size:96WELLS

Human ACACA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-97%	89-96%	89-103%	96-104%
  EDTA Plasma (n=5)	97-104%	79-88%	89-96%	78-98%
  Heparin Plasma (n=5)	83-101%	87-101%	96-105%	80-96%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Carboxylase Alpha (ACACa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Acetyl Coenzyme A Carboxylase Alpha (ACACa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	84-91	87
  EDTA Plasma (n=5)	96-103	99
  Heparin Plasma(n=5)	84-101	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetyl Coenzyme A Carboxylase Alpha (ACACa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetyl Coenzyme A Carboxylase Alpha (ACACa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ACACA Microplate: 96 Wells (12 x 8 Well strips)
  • ACACA Lyophilized Standard: 2
  • 100X Biotinylated ACACA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.09 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Acetyl Coenzyme A Carboxylase Alpha (ACACa). No significant cross-reactivity or interference between Acetyl Coenzyme A Carboxylase Alpha (ACACa) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ACACA
• Gene Full Name: acetyl-CoA carboxylase alpha
• Alias Symbols: ACAC;ACACAD;ACC;ACC1;ACCA;ACC-alpha;acetyl-CoA carboxylase 1;acetyl-Coenzyme A carboxylase alpha.
• NCBI Gene Id: 31
• Protein Name: Acetyl-CoA carboxylase 1
• Description of Target: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
• Uniprot ID: Q13085
• Protein Accession #: NP_942131.1
• Nucleotide Accession #: NM_198834.2
• Protein Size (# AA): 2346