ACACA ELISA Kit (Bovine) (OKEH03918)

Catalog No: OKEH03918

Size:96T

Bovine ACACA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ACACA ELISA Kit (Bovine) (OKEH03918) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ACACA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ACACA and incubated. The ACACA found in the sample or standards competes with the biotinylated ACACA for limited binding sites on the immobilized anti-human ACACA antibody. Excess unbound biotinylated ACACA and sample or standard ACACA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ACACA captured in the well and inversely proportional to the amount of ACACA which was contained in the sample or standard.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Component:
  • Anti-ACACA Microplate: 96 Wells (12 x 8 Well strips)
  • ACACA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X ACACA-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 29 pg/mL
• Specificity: Natural and recombinant Bovine Acetyl-CoA carboxylase 1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: ACACA
• Gene Full Name: acetyl-CoA carboxylase alpha
• Alias Symbols: ACC1;ACCA;ACC-alpha;acetyl-CoA carboxylase 1;acetyl-coenzyme A carboxylase alpha.
• NCBI Gene Id: 281590
• Protein Name: Q9TTS3
• Description of Target: Acetyl-CoA carboxylase 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q9TTS3
• Protein Accession #: NP_776649.1
• Nucleotide Accession #: NM_174224.2
• Protein Size (# AA): 2346
• Molecular Weight: 2346 kDa