ABP1 ELISA Kit (Human) (OKCD06630)

Catalog No: OKCD06630

Size:96WELLS

ABP1 ELISA Kit (Human) (OKCD06630) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Binding Protein 1 (ABP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Binding Protein 1 (ABP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Binding Protein 1 (ABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amiloride Binding Protein 1 (ABP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ABP1 Microplate: 96 Wells (12 x 8 Well strips)
  • ABP1 Lyophilized Standard: 2
  • 100X ABP1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.128ng/mL

Target Info

• Gene Symbol: AOC1
• Gene Full Name: amine oxidase copper containing 1
• Alias Symbols: ABP;ABP1;amiloride binding protein 1 (amine oxidase (copper-containing));amiloride-binding protein 1;amiloride-sensitive amine oxidase;amiloride-sensitive amine oxidase [copper-containing];amine oxidase copper domain-containing protein 1;DAO;DAO1;diamine oxidase;histaminase;KAO;kidney amine oxidase.
• NCBI Gene Id: 26
• Protein Name: Amiloride-sensitive amine oxidase [copper-containing]
• Description of Target: Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function.
• Uniprot ID: P19801
• Protein Accession #: NP_001082.2
• Nucleotide Accession #: NM_001091.3