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Catalog No: OKEH06833
Size:96T
Price: $525.00
SKU
OKEH06833
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Abcg2 ELISA Kit (Rat) (OKEH06833) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abcg2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Abcg2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abcg2 captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into matrix = 89%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <4.3%
Inter-Assay CV <9.2%
ELISA Kit Component
ComponentAmount
Abcg2 Microplate96 Wells (12 x 8 Well strips)
Abcg2 Lyophilized Standard2 x 10 ng
100X Abcg2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.081 ng/mL
SpecificityNatural and recombinant Rat ATP-binding cassette sub-family G member 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAbcg2
Gene Full NameATP binding cassette subfamily G member 2
Alias SymbolsATP binding cassette subfamily G member 2 (Junior blood group);ATP-binding cassette protein G2;ATP-binding cassette sub-family G member 2;ATP-binding cassette, subfamily G (WHITE), member 2;ATP-binding cassette, sub-family G (WHITE), member 2;Bcrp;BCRP1;breast cancer resistance protein 1 homolog;broad substrate specificity ATP-binding cassette transporter ABCG2;urate exporter.
NCBI Gene Id312382
Protein NameQ80W57
Description of TargetATP-binding cassette sub-family G member 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q80W57
Protein Accession #NP_852046
Nucleotide Accession #NM_181381
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