ABCG2 ELISA Kit (Rat) (OKCD02295)

Catalog No: OKCD02295

Size:96WELLS

Rat ABCG2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter G2 (ABCG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter G2 (ABCG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter G2 (ABCG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter G2 (ABCG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter G2 (ABCG2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter G2 (ABCG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ABCG2 Microplate: 96 Wells (12 x 8 Well strips)
  • ABCG2 Lyophilized Standard: 2
  • 100X Biotinylated ABCG2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.128 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter G2 (ABCG2). No significant cross-reactivity or interference between ATP Binding Cassette Transporter G2 (ABCG2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Abcg2
• Gene Full Name: ATP binding cassette subfamily G member 2
• Alias Symbols: ATP binding cassette subfamily G member 2 (Junior blood group);ATP-binding cassette protein G2;ATP-binding cassette sub-family G member 2;ATP-binding cassette, subfamily G (WHITE), member 2;ATP-binding cassette, sub-family G (WHITE), member 2;Bcrp;BCRP1;breast cancer resistance protein 1 homolog;broad substrate specificity ATP-binding cassette transporter ABCG2;urate exporter.
• NCBI Gene Id: 312382
• Protein Name: ATP-binding cassette sub-family G member 2
• Description of Target: High-capacity urate exporter functioning in both renal and extrarenal urate excretion. Plays a role in porphyrin homeostasis as it is able to mediates the export of protoporhyrin IX (PPIX) both from mitochondria to cytosol and from cytosol to extracellular space, and cellular export of hemin, and heme. Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain (By similarity).
• Uniprot ID: Q80W57
• Protein Accession #: NP_852046.1
• Nucleotide Accession #: NM_181381.2
• Protein Size (# AA): 657