ABCG2 ELISA Kit (Mouse) (OKEH04395)

Catalog No: OKEH04395

Size:96T

Mouse ABCG2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Abcg2 ELISA Kit (Mouse) (OKEH04395) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Abcg2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Abcg2 and incubated. The found in the sample or standards competes with the biotinylated Abcg2 for limited binding sites on the immobilized anti-Mouse Abcg2 antibody. Excess unbound biotinylated Abcg2 and sample or standard Abcg2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Abcg2 captured in the well and inversely proportional to the amount of Abcg2 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 7.6% (n = 20)
  Mean Inter-assay CV%: <= 10.1% (n = 20)
• ELISA Kit Component:
  • Anti-Abcg2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Abcg2 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Abcg2 Biotinylated Complex: 1 x 60 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.165 ng/mL
• Specificity: Natural and recombinant Mouse ATP-binding cassette sub-family G member 2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Abcg2
• Gene Full Name: ATP binding cassette subfamily G member 2 (Junior blood group)
• Alias Symbols: ABC15;ABCP;AI428558;ATP-binding cassette sub-family G member 2;ATP-binding cassette, sub-family G (WHITE), member 2;Bc;BCRP;Bcrp1;breast cancer resistance protein 1 homolog;broad substrate specificity ATP-binding cassette transporter ABCG2;mitoxantrone resistance protein 1;MXR;MXR1;urate exporter.
• NCBI Gene Id: 26357
• Protein Name: Q7TMS5
• Description of Target: ATP-binding cassette sub-family G member 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q7TMS5
• Protein Accession #: NP_036050.1
• Nucleotide Accession #: NM_011920.3
• Protein Size (# AA): 657
• Molecular Weight: 73 KDa