ABCC6 ELISA Kit (Mouse) (OKCD02336)

Catalog No: OKCD02336

Size:96WELLS

Mouse ABCC6 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C6 (ABCC6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C6 (ABCC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C6 (ABCC6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter C6 (ABCC6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter C6 (ABCC6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter C6 (ABCC6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ABCC6 Microplate: 96 Wells (12 x 8 Well strips)
  • ABCC6 Lyophilized Standard: 2
  • 100X Biotinylated ABCC6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.124 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter C6 (ABCC6). No significant cross-reactivity or interference between ATP Binding Cassette Transporter C6 (ABCC6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Abcc6
• Gene Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 6
• Alias Symbols: Abcc1b;ATP-binding cassette sub-family C member 6;ATP-binding cassette, sub-family C, member 6;DCC;Dysca;dyscalc;Dyscalc1;Mr;Mrp6;multidrug resistance-associated protein 6.
• NCBI Gene Id: 27421
• Protein Name: Multidrug resistance-associated protein 6
• Description of Target: May participate directly in the active transport of drugs into subcellular organelles or influence drug distribution indirectly.
• Uniprot ID: Q9R1S7
• Protein Accession #: NP_061265.2
• Nucleotide Accession #: NM_018795.2
• Protein Size (# AA): 1498