ABCC5 Antibody (OAEB01860)

Catalog No: OAEB01860 (Formerly GWB-8B6BE5)

Size:100UG

OAEB01860 (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB01860 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results with antigen retrieval at pH9.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Porcine|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: P-IHC, WB
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: ABCC5
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Dog, Pig, Cow
• Peptide Sequence: KDIDIGKEYIIP-C
• Concentration: 0.5 mg/ml
• Specificity: This antibody is expected to recognize both reported isoforms (NP_005679.2; NP_001018881.1).
• Application Info: P-ELISA: Antibody detection limit dilution 1:32000
  WB: Approx. 150kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 161kDa according to NP_005679.2). Recommended concentration: 0.5-1.5 ug/ml.
  IHC: In paraffin embedded Human Kidney shows membranous staining in DCT. Paraffin embedded Human Liver. Recommended concentration: 4-6 ug/ml.
• Reference: * Meyer Zu Schwabedissen HE, Grube M, Heydrich B, Linnemann K, Fusch C, Kroemer HK, Jedlitschky G.; Expression, localization, and function of MRP5 (ABCC5), a transporter for cyclic nucleotides, in human placenta and cultured human trophoblasts: effects of gestational age and cellular differentiation; Am J Pathol. 2005 Jan;166(1):39-48.; Pubmed 15631998

Target Info

• Gene Symbol: Abcc5|ABCC5
• Gene Full Name: ATP binding cassette subfamily C member 5|ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• Alias Symbols: 2900011L11Rik;ABC33;Abcc;Abcc5a;Abcc5b;AI132311;ATP-binding cassette sub-family C (CFTR/MRP) member 5a;ATP-binding cassette sub-family C member 5;ATP-binding cassette, subfamily C (CFTR/MRP), member 5;ATP-binding cassette, sub-family C (CFTR/MRP), member 5;ATP-binding cassette, sub-family C (CFTR/MRP), member 5a;ATP-binding cassette, sub-family C (CFTR/MRP), member 5b;ATP-binding cassette, sub-family C, member 5;canalicular multispecific organic anion transporter C;EST277145;MOATC;MOAT-C;Mr;Mrp5;multidrug resistance-associated protein 5;multi-specific organic anion transporter C;pABC11;SMRP.
• NCBI Gene Id: 10057|116721|27416
• Protein Name: Multidrug resistance-associated protein 5
• Uniprot ID: O15440
• Protein Accession #: NP_005679.2
• Molecular Weight: 161; 23.7