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Catalog No: OCOA13436
Size:20UG
Price: $275.00
SKU
OCOA13436
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ABCC3 Protein Lysate (OCOA13436)

Datasheets/ManualsPrintable datasheet for ABCC3 Protein Lysate (OCOA13436)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionABCC3 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolABCC3
Gene Full NameATP binding cassette subfamily C member 3
Alias SymbolsABC31;ATP-binding cassette sub-family C member 3;ATP-binding cassette, sub-family C (CFTR/MRP), member 3;canalicular multispecific organic anion transporter 2;canicular multispecific organic anion transporter;cMOAT2;EST90757;MLP2;MOAT-D;MRP3;multidrug resistance associated protein;multidrug resistance-associated protein 3;multi-specific organic anion transporter D.
NCBI Gene Id8714
Protein NameNM_003786, NP_003777, O15438
Description of TargetCanalicular multispecific organic anion transporter 2, canalicular multispecific organic anion transporter 2 isoform 1 [Homo sapiens], Homo sapiens ATP binding cassette subfamily C member 3 (ABCC3), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/O15438
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_003777
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_003786
Molecular Weight169.2 kDa
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