ABCC3 ELISA Kit (Human) (OKEH07594)

Catalog No: OKEH07594

Size:96T

Human ABCC3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	106-114% (n = 20)	91-101% (n = 20)	110-120% (n = 20)	93-103% (n = 20)
  EDTA plasma(n=5)	100-109% (n = 20)	101-111% (n = 20)	93-102% (n = 20)	100-112% (n = 20)
  heparin plasma(n=5)	82-92% (n = 20)	81-81% (n = 20)	103-111% (n = 20)	97-108% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ABCC3 ELISA Kit (Human) (OKEH07594) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC3 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.9% (n = 20)
  Mean Inter-assay CV%: <=9.7% (n = 20)
• ELISA Kit Component:
  • ABCC3 Microplate: 96 Wells (12 x 8 Well strips)
  • ABCC3 Lyophilized Standard: 2
  • 100X Biotinylated ABCC3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes.
  Subcellular Location: Membrane Multi-pass membrane protein
  Unigene: Hs.463421
  SMR: O15438
  String: 9606.ENSP00000285238
  KEGG: hsa:8714
  MIM: 604323
  Pfam: PF00664
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 21pg/mL
• Specificity: Natural and recombinant Human Canalicular multispecific organic anion transporter 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ABCC3
• Gene Full Name: ATP binding cassette subfamily C member 3
• Alias Symbols: ABC31;ATP-binding cassette sub-family C member 3;ATP-binding cassette, sub-family C (CFTR/MRP), member 3;canalicular multispecific organic anion transporter 2;canicular multispecific organic anion transporter;cMOAT2;EST90757;MLP2;MOAT-D;MRP3;multidrug resistance associated protein;multidrug resistance-associated protein 3;multi-specific organic anion transporter D.
• NCBI Gene Id: 8714
• Protein Name: O15438
• Description of Target: Canalicular multispecific organic anion transporter 2
• Uniprot ID: https://www.uniprot.org/uniprot/O15438
• Protein Accession #: NP_001137542.1
• Nucleotide Accession #: NM_001144070.1