20% off all ELISAs • Two for the price of one on select antibodies.

Don’t miss out on these great offers! Learn More Here.

Catalog No: OKCD02335
Price: $675.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
Please contact customer service when ordering 10+ kits.

Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for ABCC3 ELISA kit (Human)
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C3 (ABCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C3 (ABCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C3 (ABCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter C3 (ABCC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter C3 (ABCC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter C3 (ABCC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
Anti-ABCC3 Microplate96 Wells (12 x 8 Well strips)
ABCC3 Lyophilized Standard2
100X Biotinylated ABCC3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Chymase 1, Mast Cell (CMA1). No significant cross-reactivity or interference between Chymase 1, Mast Cell (CMA1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolABCC3
Gene Full NameATP binding cassette subfamily C member 3
Alias SymbolsABC31;ATP-binding cassette sub-family C member 3;ATP-binding cassette, sub-family C (CFTR/MRP), member 3;canalicular multispecific organic anion transporter 2;canicular multispecific organic anion transporter;cMOAT2;EST90757;MLP2;MOAT-D;MRP3;multidrug resistance associated protein;multidrug resistance-associated protein 3;multi-specific organic anion transporter D.
NCBI Gene Id8714
Protein NameO15438
Description of TargetCanalicular multispecific organic anion transporter 2
Uniprot ID
Protein Accession #NP_001137542.1
Nucleotide Accession #NM_001144070.1
Protein Size (# AA)1527
Write Your Own Review
You're reviewing:ABCC3 ELISA kit (Human)
Your Rating
We found other products you might like!