ABCC3 Antibody - middle region (OAAB00150)

Catalog No: OAAB00150

Size:400ul

All lanes : Anti-ABCC3 Antibody (Center) (Cat.# OAAB00150) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 169 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ABCC3 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCC3 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

ABCC3 Antibody (Center) (Cat. #OAAB00150) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid; PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity).
  
  Cellular Location: Membrane; Multi-pass membrane protein.
  
  Tissue Location: Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ABCC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-925 amino acids from the Central region of human ABCC3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that ABCC3 is expressed in MDA-MB435

• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: ABCC3
• Alias Symbols: MLP2, MRP3, ABC31, MOAT-D, cMOAT2, EST90757
• NCBI Gene Id: 8714
• Protein Name: Canalicular multispecific organic anion transporter 2
• Description of Target: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
• Uniprot ID: O15438
• Protein Accession #: NP_003777.2
• Molecular Weight: 169343 Da