ABCC11 ELISA Kit (Human) (OKEH08793)

Catalog No: OKEH08793

Size:96T

Human ABCC11 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	84-92% (n = 20)	106-116% (n = 20)	96-106% (n = 20)	95-105% (n = 20)
  EDTA plasma(n=5)	117-127% (n = 20)	108-117% (n = 20)	107-117% (n = 20)	87-96% (n = 20)
  heparin plasma(n=5)	103-114% (n = 20)	110-120% (n = 20)	92-92% (n = 20)	105-115% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ABCC11 ELISA Kit (Human) (OKEH08793) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCC11 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.3% (n = 20)
  Mean Inter-assay CV%: <=8.9% (n = 20)
• ELISA Kit Component:
  • ABCC11 Microplate: 96 Wells (12 x 8 Well strips)
  • ABCC11 Lyophilized Standard: 2
  • 100X Biotinylated ABCC11 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Participates in physiological processes involving bile acids, conjugated steroids and cyclic nucleotides (PubMed:12764137, PubMed:15537867). Enhances the cellular extrusion of cAMP and cGMP (PubMed:12764137, PubMed:15537867). Stimulates the ATP-dependent uptake of a range of physiological and synthetic lipophilic anions, including the glutathione S-conjugates leukotriene C4 and dinitrophenyl S-glutathione, steroid sulfates such as dehydroepiandrosterone 3-sulfate (DHEAS) and estrone 3-sulfate, glucuronides such as estradiol 17-beta-D-glucuronide (E(2)17betaG), the monoanionic bile acids glycocholate and taurocholate, and methotrexate (PubMed:15537867, PubMed:25896536). Probably functions to secrete earwax (PubMed:16444273, PubMed:19383836). Required for the secretion of components contributing to axillary odor formation (PubMed:19710689).
  Subcellular Location: Cell membrane Multi-pass membrane protein Vacuole membrane Cytoplasmic vesicle membrane
  Unigene: Hs.652267
  SMR: Q96J66
  String: 9606.ENSP00000349017
  KEGG: hsa:85320
  MIM: 117800
  Pfam: PF00664
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.39ng/mL
• Specificity: Natural and recombinant Human ATP-binding cassette sub-family C member 11
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ABCC11
• Gene Full Name: ATP binding cassette subfamily C member 11
• Alias Symbols: ATP-binding cassette protein C11;ATP-binding cassette sub-family C member 11;ATP-binding cassette transporter MRP8;ATP-binding cassette transporter sub-family C member 11;ATP-binding cassette, sub-family C (CFTR/MRP), member 11;EWWD;MRP8;multidrug resistance-associated protein 8;multi-resistance protein 8;WW.
• NCBI Gene Id: 85320
• Protein Name: Q96J66
• Description of Target: ATP-binding cassette sub-family C member 11
• Uniprot ID: https://www.uniprot.org/uniprot/Q96J66
• Protein Accession #: NP_115972.2
• Nucleotide Accession #: NM_032583.3