ABCC1 Polyclonal Antibody (OABB00587)

Catalog No: OABB00587 (Formerly GWB-BBP509)

Size:100UG

Lane1:Jurkat Cell Lysate
Lane2:CEM Cell Lysate
Lane3:A549 Cell Lysate

IHC(P):Human Lung Cancer Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Multidrug resistance-associated protein 1 (MRP1) is a protein that in humans is encoded by the ABCC1 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown.
  Background: ABCC1(ATP-BINDING CASSETTE, SUBFAMILY C, MEMBER 1),also called MRP1 or MRP, is a protein that in humans is encoded by the ABCC1 gene. The ABCC1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. The ABCC1 is a plasma membrane drug-efflux pump.The ABCC1 gene is mapped to chromosome 16p13.1 by isotopic in situ hybridization. ABCC1 functions as a multispecific organic anion transporter, with oxidized glutathione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates and it also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Blocking ABCC1 efflux pumps increases the susceptibility of the cells to the toxic effects of unconjugated bilirubin, suggesting that ABCC1 is an important protector in this scenario. ABCC1 clears beta-amyloid from brains of mice and can be activated by thiethylperazine.And Chemotaxis assays show that ABCC1-mediated transport of S1P influenced mast-cell migration, but not mast-cell degranulation.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: Mouse, Rat: By Heat
• Reference: 1. Cole, S. P. C., Bhardwaj, G., Gerlach, J. H., Mackie, J. E., Grant, C. E., Almquist, K. C., Stewart, A. J., Kurz, E. U., Duncan, A. M. V., Deeley, R. G. Overexpression of a transporter gene in a multidrug-resistant human lung cancer cell line. Science 258: 1650-1654, 1992. 2. Conrad, S., Kauffmann, H.-M., Ito, K., Deeley, R. G., Cole, S. P. C., Schrenk, D. Identification of human multidrug resistance protein 1 (MRP1) mutations and characterization of a G671V substitution. J. Hum. Genet. 46: 656-663, 2001. 3. Grant, C. E., Kurz, E. U., Cole, S. P. C., Deeley, R. G. Analysis of the intron-exon organization of the uman multidrug-resistance protein gene (MRP) and alternative splicing of its mRNA. Genomics 45: 368-378, 1997.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Multidrug resistance-associated protein 1(ABCC1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: ABCC1
• Gene Full Name: ATP binding cassette subfamily C member 1
• Alias Symbols: ABC29;ABCC;ATP-binding cassette sub-family C member 1;ATP-binding cassette transporter variant ABCC1delta-ex13;ATP-binding cassette transporter variant ABCC1delta-ex13&14;ATP-binding cassette transporter variant ABCC1delta-ex25;ATP-binding cassette transporter variant ABCC1delta-ex25&26;ATP-binding cassette, sub-family C (CFTR/MRP), member 1;DFNA77;glutathione S-conjugate export pump;glutathione-S-conjugate-translocating ATPase ABCC1;GS-X;leukotriene C(4) transporter;LTC4 transporter;MRP;MRP1;multidrug resistance-associated protein 1.
• NCBI Gene Id: 4363
• Protein Name: Multidrug resistance-associated protein 1
• Description of Target: Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. Hydrolyzes ATP with low efficiency.
• Uniprot ID: P33527
• Molecular Weight: 171591 MW