ABCC1 ELISA Kit (Rat) (OKEH03279)

Catalog No: OKEH03279

Size:96T

Rat ABCC1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ABCC1 ELISA Kit (Rat) (OKEH03279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abcc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Abcc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abcc1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: <= 5.3%
• ELISA Kit Component:
  • Anti-Abcc1 Microplate: 96 Wells (12 x 8 Well strips)
  • Abcc1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Abcc1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Rat Multidrug resistance-associated protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Abcc1
• Gene Full Name: ATP binding cassette subfamily C member 1
• Alias Symbols: Abcc1a;ATP-binding cassette sub-family C ( CFTR/MRP) member 1a;ATP-binding cassette sub-family C member 1;ATP-binding cassette, subfamily C (CFTR/MRP), member 1;ATP-binding cassette, sub-family C (CFTR/MRP), member 1;ATP-binding cassette, sub-family C, member 1-like;Avcc1a;glutathione-S-conjugate-translocating ATPase ABCC1;leukotriene C(4) transporter;LTC4 transporter;Mrp;Mrp1;multidrug resistance protein 1;multidrug resistance-associated protein 1;multidrug resistance-associated protein 1-like;multiple drug resistance-associated protein.
• NCBI Gene Id: 24565
• Protein Name: Q8CG09
• Description of Target: Multidrug resistance-associated protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q8CG09
• Protein Accession #: NP_071617.2
• Nucleotide Accession #: NM_022281.2
• Protein Size (# AA): 1532
• Molecular Weight: 171 kDa