ABCC1 ELISA Kit (Human) (OKCA02290)

Catalog No: OKCA02290

Size:96 Wells

Human ABCC1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	89	85-93
  1:2	87	82-93
  1:4	90	87-93
  1:8	87	82-93

• ELISA Kit Principle: Aviva Systems Biology ABCC1 ELISA Kit (Human) (OKCA02290) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCC1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCC1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard ABCC1 captured in the well.
• ELISA Kit Range: 15.6 - 1,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	90	87-93
  EDTA Plasma (n=4)	88	85-91

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • ABCC1 Microplate: 96 Wells (12 x 8 Well strips)
  • ABCC1 Lyophilized Standard: 2
  • 100X Biotinylated ABCC1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ABCC1
• Alias Symbols: ABC29, ABCC, DKFZp686N04233, DKFZp781G125, GS-X, MRP, MRP1, ATP-binding cassette, sub-family C, member 1, LTC4 transporter, leukotriene C(4) transporter, multidrug resistance protein, multiple drug resi
• NCBI Gene Id: 281588
• Protein Name: Multidrug resistance-associated protein 1
• Description of Target: Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs. May protect milk against xenobiotics. Hydrolyzes ATP with low efficiency.
• Uniprot ID: Q8HXQ5
• Protein Accession #: NP_776648
• Nucleotide Accession #: NM_174223