ABCB5 Antibody - N-terminal region (OAAB16481)

Catalog No: OAAB16481

Size:400ul

Western blot analysis of ABCB5 Antibody (N-term) (Cat.# OAAB16481) in A375, K562, A2058 and HL-60 cell line lysates (35ug/lane). ABCB5 (arrow) was detected using the purified Pab.

Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# OAAB16481 on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver

Flow Cytometry using ABCB5 Antibody (N-Term) Cat.# OAAB16481 on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit (Jackson ImmunoResearch) as a secondary antibody.?Analysis was done on an FC500 flow cytometer. Data courtesy of Dr. Steve Reuland, University of Colorado, Denver

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Precautions: ABCB5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas
  Cellular Location: Cell membrane; Multi-pass membrane protein.
  Tissue Location: Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: FC: 1:25
  WB: 1:2000
  WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: ABCB5
• Alias Symbols: ABCB5beta, EST422562, ABCB5alpha
• NCBI Gene Id: 340273
• Protein Name: ATP-binding cassette sub-family B member 5
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q2M3G0
• Protein Accession #: NP_001157413.1
• Molecular Weight: 89832 Da