Catalog No: OKCD07594
Size:96WELLS
Price: $650.00
SKU
OKCD07594
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for ABCB1B ELISA Kit (Mouse) (OKCD07594) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Permeability Glycoprotein (Pgp). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Permeability Glycoprotein (Pgp). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Permeability Glycoprotein (Pgp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Permeability Glycoprotein (Pgp) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Permeability Glycoprotein (Pgp) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Permeability Glycoprotein (Pgp) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.054ng/mL |
Gene Symbol | Abcb1b |
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Gene Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 1B |
Alias Symbols | Abcb;Abcb1;ATP-binding cassette sub-family B member 1;ATP-binding cassette sub-family B member 1B;ATP-dependent translocase ABCB1;md;mdr;Mdr1;Mdr1b;multidrug resistance protein 1;multidrug resistance protein 1B;P glycoprotein 1;P-glycoprotein 1;Pgy;Pgy-;Pgy1;Pgy-1;phospholipid transporter ABCB1. |
NCBI Gene Id | 18669 |
Protein Name | Multidrug resistance protein 1B |
Description of Target | Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. |
Uniprot ID | P06795 |
Protein Accession # | NP_035205.1 |
Nucleotide Accession # | NM_011075.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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