ABCA4 ELISA Kit (Human) (OKEH07592)

Catalog No: OKEH07592

Size:96T

Human ABCA4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	103-116% (n = 20)	97-107% (n = 20)	108-116% (n = 20)	95-105% (n = 20)
  EDTA plasma(n=5)	99-108% (n = 20)	91-103% (n = 20)	87-97% (n = 20)	97-107% (n = 20)
  heparin plasma(n=5)	86-98% (n = 20)	105-114% (n = 20)	91-103% (n = 20)	84-94% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ABCA4 ELISA Kit (Human) (OKEH07592) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABCA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ABCA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABCA4 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.3% (n = 20)
  Mean Inter-assay CV%: <=9.7% (n = 20)
• ELISA Kit Component:
  • ABCA4 Microplate: 96 Wells (12 x 8 Well strips)
  • ABCA4 Lyophilized Standard: 2
  • 100X Biotinylated ABCA4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Catalyzes the translocation of specific phospholipids from the extracellular/lumenal to the cytoplasmic leaflet of membrane coupled to the hydrolysis of ATP (PubMed:24097981). Transports preferentially phosphatidylethanolamine (PubMed:24097981). In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.
  Subcellular Location: Membrane Multi-pass membrane protein Endoplasmic reticulum Localized to outer segment disk edges of rods and cones, with around one million copies/photoreceptor.
  Unigene: Hs.416707
  SMR: P78363
  String: 9606.ENSP00000359245
  KEGG: hsa:24
  MIM: 153800
  Pfam: PF00005
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 38pg/mL
• Specificity: Natural and recombinant Human Retinal-specific phospholipid-transporting ATPase ABCA4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ABCA4
• Gene Full Name: ATP binding cassette subfamily A member 4
• Alias Symbols: ABC10;ABCR;ARMD2;ATP binding cassette transporter;ATP-binding cassette sub-family A member 4;ATP-binding cassette transporter, retinal-specific;ATP-binding cassette, sub-family A (ABC1), member 4;ATP-binding transporter, retina-specific;CORD3;FFM;photoreceptor rim protein;retinal-specific ATP-binding cassette transporter;retinal-specific phospholipid-transporting ATPase ABCA4;retina-specific ABC transporter;RIM ABC transporter;RIM protein;RMP;RP19;stargardt disease protein;STGD;STGD1.
• NCBI Gene Id: 24
• Protein Name: P78363
• Description of Target: Retinal-specific ATP-binding cassette transporter
• Uniprot ID: https://www.uniprot.org/uniprot/P78363
• Protein Accession #: NP_000341.2
• Nucleotide Accession #: NM_000350.2