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Catalog No: OKCD07241
Size:96WELLS
Price: $750.00
SKU
OKCD07241
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Datasheets/ManualsPrintable datasheet for ABCA1 ELISA Kit (Human) (OKCD07241)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter A1 (ABCA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter A1 (ABCA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter A1 (ABCA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP Binding Cassette Transporter A1 (ABCA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter A1 (ABCA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter A1 (ABCA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
ABCA1 Microplate96 Wells (12 x 8 Well strips)
ABCA1 Lyophilized Standard2
100X ABCA1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.055ng/mL
Gene SymbolABCA1
Gene Full NameATP binding cassette subfamily A member 1
Alias SymbolsABC1;ABC-1;ATP-binding cassette sub-family A member 1;ATP-binding cassette transporter 1;ATP-binding cassette transporter A1;ATP-binding cassette, sub-family A (ABC1), member 1;CERP;cholesterol efflux regulatory protein;HDLCQTL13;HDLDT1;HPALP1;membrane-bound;phospholipid-transporting ATPase ABCA1;TGD.
NCBI Gene Id19
Protein NameATP-binding cassette sub-family A member 1
Description of TargetcAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
Uniprot IDO95477
Protein Accession #NP_005493.2
Nucleotide Accession #NM_005502.3
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