AAGAB ELISA Kit (Mouse) (OKEH08055)

Catalog No: OKEH08055

Size:96T

Mouse AAGAB ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology AAGAB ELISA Kit (Mouse) (OKEH08055) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AAGAB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AAGAB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AAGAB captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.6% (n = 20)
  Mean Inter-assay CV%: <=5.2% (n = 20)
• ELISA Kit Component:
  • AAGAB Microplate: 96 Wells (12 x 8 Well strips)
  • AAGAB Lyophilized Standard: 2
  • 100X Biotinylated AAGAB Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May be involved in endocytic recycling of growth factor receptors such as EGFR.
  Subcellular Location: Cytoplasm Cytosol
  Unigene: Mm.34338
  SMR: Q8R2R3
  String: 10090.ENSMUSP00000048595
  KEGG: mmu:66939
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.093ng/mL
• Specificity: Natural and recombinant Mouse Alpha- and gamma-adaptin-binding protein p34
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Aagab
• Gene Full Name: alpha- and gamma-adaptin binding protein
• Alias Symbols: 2310007F21Rik;alpha- and gamma-adaptin-binding protein p34;p34.
• NCBI Gene Id: 66939
• Protein Name: Q8R2R3
• Description of Target: Alpha- and gamma-adaptin-binding protein p34
• Uniprot ID: https://www.uniprot.org/uniprot/Q8R2R3
• Protein Accession #: NP_080133.1
• Nucleotide Accession #: NM_025857.2