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Catalog No: OKEH02241
Size:96T
Price: $525.00
SKU
OKEH02241
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02241
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AADAT ELISA Kit (Human) (OKEH02241) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AADAT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AADAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AADAT captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 10.3% (n = 20)
ELISA Kit Component
ComponentAmount
AADAT Microplate96 Wells (12 x 8 Well strips)
AADAT Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated AADAT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAADAT
Gene Full Nameaminoadipate aminotransferase
Alias Symbols2-aminoadipate aminotransferase;2-aminoadipate transaminase;alpha-aminoadipate aminotransferase;KAT/AadAT;KAT2;KATII;KYAT2;kynurenine aminotransferase II;kynurenine/alpha-aminoadipate aminotransferase, mitochondrial;kynurenine--oxoglutarate aminotransferase II;kynurenine--oxoglutarate transaminase 2;kynurenine--oxoglutarate transaminase II;L kynurenine/alpha aminoadipate aminotransferase.
NCBI Gene Id51166
Protein NameQ8N5Z0
Description of TargetKynurenine/alpha-aminoadipate aminotransferase, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/Q8N5Z0
Protein Accession #NP_001273611.1
Nucleotide Accession #NM_001286682.1
Protein Size (# AA)425
Molecular Weight47 kDa
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