AADAT Antibody - N-terminal region (ARP43534_T100)

Catalog No: ARP43534_T100

WB Suggested Anti-AADAT Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Protein is processed to mature 44 kDa and the protein may be modified by succinyl lysine at several residues.

Human kidney

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AADAT
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Horse: 86%; Human: 100%; Mouse: 93%; Pig: 79%; Rabbit: 100%; Rat: 85%; Zebrafish: 86%
• Peptide Sequence: Synthetic peptide located within the following region: AVITVENGKTIQFGEEMMKRALQYSPSAGIPELLSWLKQLQIKLHNPPTI
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-AADAT (ARP43534_T100) antibody is Catalog # AAP43534 (Previous Catalog # AAPP11526)
• Enhanced Validation:

  Relative Expression (Western Blot)

• Reference: Goh,D.L., (2002) Mol. Genet. Metab. 76 (3), 172-180

Target Info

• Gene Symbol: AADAT
• Gene Full Name: Aminoadipate aminotransferase
• Alias Symbols: KAT2, KATII, KYAT2
• NCBI Gene Id: 51166
• Protein Name: Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
• Description of Target: AADAT is a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties.This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Two alternative transcripts encoding the same isoform have been identified, however, additional alternative transcripts and isoforms may exist.
• Uniprot ID: Q8N5Z0
• Protein Accession #: NP_057312
• Nucleotide Accession #: NM_016228
• Protein Size (# AA): 425
• Molecular Weight: 47 kDa