A2M Protein (OPPA01428)

Catalog No: OPPA01428 (Formerly GWB-39F186)

Size:200UG

Product Info

• Product Format: Lyophilized 5mM potassium phosphate buffer (pH 6.5) and 1:1 (w/w) of glycine
• Host: Human Plasma
  Human Virus Test: Serum from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBV.
• Reconstitution and Storage: It is recommended to reconstitute the lyophilized A2M in sterile 18M-omega-cm H₂O. Lyophilized A2M protein, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization A2M can be stored at 4°C for up to 4 weeks. For long-term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze/thaw cycles.
• Purity: > 95% as determined by Analysis by SDS-PAGE

Target Info

• Gene Symbol: A2M
• Alias Symbols: A2MD, CPAMD5, FWP007, S863-7
• NCBI Gene Id: 2
• Protein Name: Alpha-2-macroglobulin
• Description of Target: Alpha-2 Macroglobulin is a serine proteases inhibitor, which inhibits coagulation by inactivating thrombin and Kallikrein, it inhibits fibrinolysis by inactivating plasmin and involved in transport. Alpha-2-Macroglobulin is a large plasma protein, which is produced by the liver, it’s composed of 4 identical subunits bound together by -S-S- bonds. A2M is able to inactivate many kinds of proteinases (including serine-, cysteine-, aspartic- and metalloproteinases). A2M has a 35 amino acid "bait" region in its structure. Proteinases bind and cleave the “bait” region become bound to A2M. Macrophage receptors recognize the proteinase-A2M complex and clear it from the system. A2M binds to and removes MMP-2 and MMP-9 (active forms of the gelatinase) from the circulation using scavenger receptors on the phagocytes. The levels of Alpha-2-macroglobulin are increased in nephrotic syndrome which is a condition where the kidneys start to leak out some of the smaller blood proteins. Due to its large size, A2-macroglobulin is retained in the bloodstream. Increased production of all proteins causes A2-macroglobulin concentration to increase. Chronic renal failure might lead to amyloid by alpha-2-macroglobulin. A2M is raised in cirrhosis, pregnancy and diabetes.
• Uniprot ID: P01023
• Protein Accession #: NP_000005.2
• Molecular Weight: 725 kDa