Catalog No: OKEH02993
Size:96T
Price: $425.00
SKU
OKEH02993
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology A2M ELISA Kit (Rat) (OKEH02993) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for A2m has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for A2m is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample A2m captured in well.
ELISA Kit Range7.8-500ng/ml
ELISA Kit Component
ComponentAmount
Anti-A2m Microplate96 Wells (12 x 8 Well strips)
A2m Lyophilized Standard2
100X Biotinylated A2m Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity4.02 ng/mL
SpecificityNatural and recombinant Rat Alpha-2-macroglobulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolA2m, LOC100911545
Gene Full Namealpha-2-macroglobulin, alpha-2-macroglobulin-like
Alias SymbolsA2m1;A2maa;A2MAC1;A2mb;alpha-2-M;alpha-2-macroglobulin;alpha-2-macroglobulin-P;Mam.
NCBI Gene Id100911545, 24153
Protein NameP06238
Description of TargetAlpha-2-macroglobulin
Uniprot IDhttps://www.uniprot.org/uniprot/P06238
Protein Accession #NP_036620.2
Nucleotide Accession #NM_012488.2
Protein Size (# AA)1472
Molecular Weight164 kDa
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