A2M ELISA Kit (Human) (OKGD00005)

Catalog No: OKGD00005

Size:96 Wells

Human A2M ELISA Kit standard curve.

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~2.5 hours
• ELISA Kit Principle: Aviva Systems Biology A2M ELISA Kit (human) (OKGD00005) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for A2M has been pre-coated onto a 96-well plate. Standards or test samples are added to the wells, incubated and washed. A horseradish peroxidase (HRP) conjugated detector antibody specific for A2M is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample A2M captured in well.
• ELISA Kit Range: 1.95 - 500 ng/ml
• ELISA Kit Component:
  • Anti-A2M Microplate: 96 Wells (12 x 8 Well strips)
  • A2M Lyophilized Standard: 2 vials
  • 100X A2M HRP-Detection Anitbody: 1 x 125 uL
  • Assay Diluent: 1 x 30 mL
  • 20X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 12 mL
  • Stop Solution: 1 x 12 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Other Biological Fluids
• Sensitivity: < 1.6 ng/ml
• Specificity:

  Species Reactivity
  Species	Cross Reactivity (%)
  Chicken	0.5%
  Dog	4%
  Mouse	1%
  Pig	11%
  Rat	4%

  Cross-Reactivity
  Antigen	Cross Reactivity (%)
  AAT	0%
  APO	0%
  C3	0%
  Fibrinogen	0%
  Haptoglobin	0%
  HDL	0%
  H. HSA	5%
  IgA	0%
  IgG	0%
  IgM	5%
  LDL	0%
  Orosomucoid	0%
  Transferrin	0%

Target Info

• Gene Symbol: A2M
• Gene Full Name: Alpha-2-macroglobulin
• Alias Symbols: A2MD, CPAMD5, FWP007, S863-7
• NCBI Gene Id: 2
• Protein Name: Alpha-2-macroglobulin
• Description of Target: The protein encoded by this gene is a protease inhibitor and cytokine transporter. It uses a bait-and-trap mechanism to inhibit a broad spectrum of proteases, including trypsin, thrombin and collagenase. It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades. Mutations in this gene are a cause of alpha-2-macroglobulin deficiency. This gene is implicated in Alzheimer's disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. A related pseudogene, which is also located on the p arm of chromosome 12, has been identified.
• Uniprot ID: P01023
• Protein Accession #: NP_000005.2
• Nucleotide Accession #: NM_000079