Catalog No: OKEH03891
Size:96 Wells
Price: $625.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology A2M ELISA Kit (Bovine) (OKEH03891) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for A2M has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for A2M is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample A2M captured in well.
ELISA Kit Range3.12 - 200ng/mL
ELISA Kit Component
Anti-A2M Microplate96 Wells (12 x 8 Well strips)
A2M Lyophilized Standard2
100X Biotinylated A2M Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity1.47 ng/mL
SpecificityNatural and recombinant Bovine Alpha-2-macroglobulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolA2M
Gene Full Namealpha-2-macroglobulin
Alias SymbolsAlpha-2-M; Alpha-2-macroglobulin
NCBI Gene Id513856
Protein NameAlpha-2-macroglobulin(Alpha-2-M)
Description of TargetIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Swissprot IdQ7SIH1
Protein Accession #NP_001103265.1
Nucleotide Accession #NM_001109795.1
Protein Size (# AA)1510
Molecular Weight168 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express A2M.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express A2M.
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