A1CF Antibody - C-terminal region (OAAB00687)

Catalog No: OAAB00687

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ACF Antibody (C-term) western blot analysis in A375 cell lysates (35ug/lane).This demonstrates the ACF antibody detected the ACF protein (arrow).

ACF Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ACF Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Tissue Location: Widely expressed with highest levels in brain, liver, pancreas, colon and spleen.
  Cellular Location: Nucleus. Endoplasmic reticulum. Cytoplasm. Note=Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum. Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Varies by lot. See vial for concentration.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: A1CF
• Alias Symbols: ACF, ASP, ACF64, ACF65, APOBEC1CF
• NCBI Gene Id: 29974
• Protein Name: APOBEC1 complementation factor
• Uniprot ID: Q9NQ94
• Protein Accession #: NP_620310
• Molecular Weight: 65 kDa