A1BG ELISA Kit (Pig) (OKEH07646)

Catalog No: OKEH07646

Size:96T

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	100-109% (n = 20)	117-127% (n = 20)	109-119% (n = 20)	105-115% (n = 20)
  EDTA plasma(n=5)	92-101% (n = 20)	82-92% (n = 20)	104-114% (n = 20)	102-112% (n = 20)
  heparin plasma(n=5)	95-104% (n = 20)	88-98% (n = 20)	99-109% (n = 20)	104-114% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology A1BG ELISA Kit (Pig) (OKEH07646) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for A1BG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for A1BG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample A1BG captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.7% (n = 20)
  Mean Inter-assay CV%: <=11.2% (n = 20)
• ELISA Kit Component:
  • A1BG Microplate: 96 Wells (12 x 8 Well strips)
  • A1BG Lyophilized Standard: 2
  • 100X Biotinylated A1BG Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
  
  Subcellular Location: Secreted
  Unigene: Ssc.18514
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Pig Alpha-1B-glycoprotein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: A1BG
• Alias Symbols: Alpha-1-B glycoprotein;Plasma protein PO2-F.
• Protein Name: P39092
• Description of Target: Alpha-1B-glycoprotein
• Uniprot ID: https://www.uniprot.org/uniprot/P39092