A1AT ELISA Kit (Mouse) (OKCD04467)

Catalog No: OKCD04467 (Formerly GWB-UFC4AT)

Size:96WELLS

Mouse A1AT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	81-104%	83-93%	86-96%	82-103%
  EDTA Plasma (n=5)	95-105%	96-103%	85-103%	90-104%
  Heparin Plasma (n=5)	84-104%	80-96%	80-94%	85-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-1-Antitrypsin (a1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	90-104	99
  EDTA Plasma (n=5)	99-105	102
  Heparin Plasma (n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Angptl3 Microplate: 96 Wells (12 x 8 Well strips)
  • Angptl3 Lyophilized Standard: 2
  • 100X Angptl3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 12.8 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Serpina1a|Serpina1d
• Gene Full Name: serine (or cysteine) peptidase inhibitor, clade A, member 1A|serine (or cysteine) peptidase inhibitor, clade A, member 1D
• Alias Symbols: Aa;AAT;Aat2;Aat-2;AI118301;alpha-1 protease inhibitor 1;alpha-1 protease inhibitor 4;alpha-1-antiproteinase;alpha-1-antitrypsin 1-1;alpha-1-antitrypsin 1-4;alpha-1-antitrypsin 2;Dom1;Dom4;DOM-7;P;PI1;PI4;serine (or cysteine) proteinase inhibitor, clade A, member 1a;serine (or cysteine) proteinase inhibitor, clade A, member 1d;serine protease inhibitor 1-1;serine protease inhibitor 1-4;serine protease inhibitor A1a;serine protease inhibitor A1d;serpin A1a;serpin A1d;Serpina1 DOM-7;Serpina1a;Spi;Spi1-1;Spi1-3;Spi1-4.
• NCBI Gene Id: 20700|20703
• Protein Name: Alpha-1-antitrypsin 1-1
• Description of Target: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.
• Uniprot ID: P07758
• Protein Accession #: NP_033269.1
• Nucleotide Accession #: NM_009243.4