Catalog No: OKCA00594
Size:96 Wells
Price: $754.00
SKU
OKCA00594
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9388-97
1:29793-103
1:48581-89
1:810286-105
ELISA Kit PrincipleAviva Systems Biology YWHAH ELISA Kit (Human) (OKCA00594) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for YWHAH has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for YWHAH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample YWHAH captured in the well.
ELISA Kit Range0.625 ng/mL-40 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9286-98
EDTA Plasma (n=4)8882-94
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level YWHAH were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level YWHAH were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
ELISA Kit Component
ComponentAmount
YWHAH Microplate96 Wells (12 x 8 Well strips)
YWHAH Lyophilized Standard2
100X Biotinylated YWHAH Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolYWHAH
Gene Full NameTyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta
Alias SymbolsYWHA1
NCBI Gene Id7533
Protein Name14-3-3 protein eta
Description of TargetAdapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
Uniprot IDQ04917
Protein Accession #NP_003396.1
Nucleotide Accession #NM_003405.3
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